Table 2. Candidate target genes in the vicinity (±50 kb) of the G12424 insertion, as numbered in Fig. 3A.
| # | Gene IDa | Orthology name andFlyBase CG#d | Drosophila gene descriptions, including stages of peak expression (PE) and embryonic in situ (eISH) expression notes (citations in Methods) |
| 1 | TC008244 (au2.g6126.t1; au3.g5375.t1) | No orthologue;best hit: goliath(gol; CG2679) | Zinc-finger transcription factor; role in mesoderm development; PE: 6–24 h embryonic and all later stages; eISH: visceral and pharyngeal muscles, stages 11–16 |
| 2 | TC007230 (au2.g6127.t1; au3.g5376.t1) | – | No homologues in Drosophila. Eleven similar genes in Tribolium. |
| 3 | TC007229 (au2.g6129.t1; au3.g5378.t1) | Ef1y (CG11901) | Translation elongation activity; PE: 0–12 h embryonic and early larval; eISH: maternal and ubiquitous, stages 1–16 |
| 4 | TC008245 (au2.g6130.t1; au3.g5379.t1) | CG3702 | Transmembrane protein; larval lethal; PE: 0–18 h embryonic and all later stages; eISH: maternal and ubiquitous, stages 1–16 |
| 5 | TC008246 (au2.g6131.t1; au3.g5380.t1) | Glycogen phosphorylase(Gly; CG7254) | Innate immune response and glycogen catabolism; PE: 0–6 and 12–24 embryonic and all later stages; eISH: rapidly degraded, ubiquitous maternal expression, later somatic muscles, stages 1–6 and 13–16 |
| 6 | TC008247b (au2.g6132.t1; au3.g5381.t1) | – | No homologues in Drosophila or Tribolium. |
| 7 | TC008248b (au2.g6132.t1; au3.g5381.t1) | – | No homologues in Drosophila or Tribolium. |
| 8 | TC007228c (au2.g6133.t1; au3.g5382.t1) | TBP-related factor (Trf;CG7562) | TFIIA-class transcription factor; PE: 0–6 h embryonic; eISH: maternal and ubiquitous, stages 1–12 |
| 9 | TC007227c (au2.g6135.t1; au3.g5384.t1) | Synaptotagmin 1 (Syt 1;CG3139) | Calcium binding; neurotransmitter transport function; PE: 12–24 h embryonic and all later stages; eISH: central nervous system, stages 13–16 |
| 10 | TC008249 (–; TC008249) | – | No homologues in Drosophila or Tribolium. |
| 11 | TC008250 (au2.g6136.t1; au3.g5385.t1) | – | No homologues in Drosophila or Tribolium. |
| 12 | TC007226 (au2.g6137.t1; au3.g5386.t1) | kekkon-3(kek3; CG4192) | Immunoglobulin-like domain; PE: 12–24 h embryonic and all later stages, including adult brain; eISH: no staining detected (with EST IP22191), stages 1–16 |
| 13 | TC007225 (–; TC007225) | – | No homologues in Drosophila or Tribolium. |
a
Genes are identified by protein prediction models from Tcas_3.0 (official gene set (OGS): TC#, shown in Fig. 3A), AUGUSTUS2 (au2, from July 2012), and AUGUSTUS3 (au3, from September 2013).
b
Indicates a gene with multiple OGS identifiers but a single Augustus model.
c
Indicates genes for which in situ hybridization was tested.
d
Based on reciprocal BLAST identification of the probable Drosophila melanogaster orthologue, or non-orthologous best hit, within the Tribolium genome browser.