Table 3. Candidate target genes in the vicinity (±50 kb) of the KT650 insertion, as numbered in Fig. 3B.
| # | Gene IDa | Orthology name and FlyBase CG#d | Drosophila gene descriptions, including stages of peak expression (PE) and embryonic in situ (eISH) expression notes (citations in Methods) |
| 1 | TC016314c (–; au3.g5845.t1) | CG7049 | Sulphatase enzyme; PE: 0–6 embryonic and pupal, expressed in many adult organs; eISH: no entry in database |
| 2 | TC016313b (au2.g6668.t1, au2.g6669.t1 (partial); au3.g5844.t1, au3.g5843.t1 (partial)) | CG11905 | Unknown molecular function; PE: pupal stages; eISH: dorsal and head epidermis, tracheal system, and posterior spiracles, stages 11–16 |
| 3 | TC016312b , c (–; au3.g5843.t1 (partial)) | CG11905 | Unknown molecular function; PE: pupal stages; eISH: dorsal and head epidermis, tracheal system, and posterior spiracles, stages 11–16 |
| 4 | TC016319 (au2.g6670.t1; au3.g5842.t1) | – | No homologues in Drosophila or Tribolium. |
| 5 | TC016311 (au2.g6671.t1; au3.g5841.t1) | Cuticular protein 73D(Cpr73D; CG9665) | Structural constituent of chitin-based cuticle; PE: late pupal stages; eISH: restricted regions of the ventral epidermis, stages 13–16 |
| 6 | TC016310 (au2.g6672.t1; au3.g5840.t1) | Odorant-binding protein 73a (Obp73a) | Pheromone binding; PE: late pupal stages; eISH: no entry in database |
| 7 | TC016320 (au2.g6673.t1; au3.g5839.t1) | CG4729 | Phospholipid/glycerol acyltransferase; PE: 12–18 h embryonic and all later stages in all tissues; eISH: maternal and ubiquitous during stages 1–10, later in the endoderm, midgut, procrystal cell, ring gland, oenocytes, and posterior spiracle during stages 9–16 |
| 8 | TC016309 (au2.g6674.t1; au3.g5838) | Gonadal (gdl; CG33756) | Gonadogenesis; PE: pupal and adult male; eISH: no entry in database |
a
Genes are identified by protein prediction models from Tcas_3.0 (official gene set (OGS): TC#, shown in Fig. 3B), Augustus2 (au2, from July 2012), and Augustus3 (au3, from September 2013).
b
Indicates a gene with multiple OGS identifiers but a single Augustus model.
c
Indicates genes for which in situ hybridization was tested.
d
Based on reciprocal BLAST identification of the probable Drosophila melanogaster orthologue, or non-orthologous best hit, within the Tribolium genome browser.