Table 1. DbpA variants differ in binding to decorin and dermatan sulfate.

		ELISA	----- Surface Plasmon Resonance -----
DbpA variant	Ligand	KD (µM)	KD (µM)	kon (105 s−1M−1)	koff (s−1)
B. burgdorferi
DbpAB31	Decorin	0.21±0.03	0.28±0.03	4.17±0.70	0.11±0.01
	Derm SO4	0.91±0.02	0.63±0.04	0.36±0.01	0.02±0.01
DbpA297	Decorin	0.14±0.06	0.30±0.08	3.51±0.86	0.10±0.01
	Derm SO4	0.78±0.18	0.50±0.01	0.52±0.02	0.02±0.01
DbpAB356	Decorin	0.95±0.12	0.84±0.04	0.66±0.33	0.06±0.01
	Derm SO4	3.68±0.21	1.91±0.87	0.44±0.04	0.07±0.03
DbpAN40-D10/E9	Decorin	0.85±0.12	0.71±0.01	0.97±0.42	0.07±0.01
	Derm SO4	3.10±0.26	2.58±0.17	0.13±0.06	0.03±0.01
B. garinii
DbpAPBr	Decorin	0.06±0.01	0.09±0.06	18.3±1.10	0.13±0.02
	Derm SO4	0.21±0.03	0.16±0.02	0.56±0.02	0.009±0.001
B. afzelii
DbpAVS461	Decorin	0.29±0.07	0.26±0.02	1.52±0.23	0.04±0.01
	Derm SO4	1.55±0.63	1.61±0.48	0.43±0.06	0.07±0.03
DbpAVS461ΔC11	Decorin	n.b a	n.b.	n.b.	n.b.
	Derm SO4	n.b.	n.b.	n.b.	n.b.

All values represent the mean ± SEM of three experiments.

^aNo binding activity was detected.