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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Nat Cell Biol. 2014 Mar 16;16(4):335–344. doi: 10.1038/ncb2920

Figure 5. Motility regulation by β-tubulin isotypes.

Figure 5

a. Sequence alignment of helix 12 (yellow) and CTTs (red) of human β-tubulin isotypes. The basic residues in TUBB1 and TUBB3 CTTs are highlighted in blue.

b. Kinesin-1 and dynein motors velocity and processivity values measured against various β-tubulin isotypes as indicated (in Δα-CTT background). Mean velocity and run length were determined as described in Fig 1.

c. Run length histograms of kinesin-1 motors moving on TUBB3 (blue) and TUBB3ΔK (red) microtubules (mean run length of 0.6 and 1.35 μm respectively). Examples of raw kymographs (inset panel; scale, 2 sec and 1.5 μm).