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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Nat Cell Biol. 2014 Mar 16;16(4):335–344. doi: 10.1038/ncb2920

Figure 6. Cross-regulation of isotype specificity by polyglutamylation.

Figure 6

a. Illustration of TUBA1A-E452C/TUBB3 heterodimer crosslinking strategy with 10E.

b. SDS-PAGE of crosslinked product of 10 and 3 glutamic acid peptides (10E and 3E) as indicated in Fig. 4.

c. Kinesin-1 motility kymographs (inlet panel) and run length histograms of TUBB3/TUBA1A- E452C and +10E (red) microtubule (mean run lengths of 0.5 and 1.5 μm respectively). Scale bars and bin size are similar to 4c.

c. The ratio of the motor velocity (red hatched), processivity (solid red) on uncrosslinked versus 10E and 3E crosslinked TUBA1A- E452C/TUBB3 microtubules is shown (mean and s.e.m; n = 3 independent experiments, see Supplementary Table 1). Absolute values are shown in Table 1.