Table 1. Motor parameters on microtubules composed of different recombinant tubulins.
Velocities shown are the mean ± s.d. (n, the total number of measurements). The mean run length was calculated using a cumulative probability function as described earlier41. The inverse cumulative probability distribution was fit to a simple exponential decay function; the errors reported here are the standard errors of the fit. For kinesin-1, -2 and dynein ‘n’ represents number of motile motors analyzed, in the case of kinesin-13 ‘n’ represents number of microtubule ends analyzed. The values are combined from 2–5 different experiments. Kinesin-13 – Chinese Hamster MCAK, PMTs – Porcine brain microtubules, YMTs – Yeast microtubules, α1a – TUBA1A, βII – TUBB2A, βIII – TUBB3, βIV – TUBB4A, βV – TUBB6, βVI – TUBB1, βVII – TUBB7, βVIII – TUBB8, ND – not determined.
| Human Kinesin-1 | Yeast Dynein | Human Kinesin-2 | C. elegans Kinesin-2 | Kinesin-13 | |||||
|---|---|---|---|---|---|---|---|---|---|
| Velocity, μm s−1 | Processivity, μm | Velocity, μm s−1 | Processivity, μm | Velocity, μm s−1 | Processivity, μm | Velocity, μm s−1 | Processivity, μm | Rate, μm min−1 | |
| pMTs | 0.53 ± 0.19 | 1.99 ± 0.02 (n=340) | 0.11 ± 0.04 | 2.1 ± 0.01 (n=312) | 1.26 ± 0.33 | 4.0 ± 0.04 (n=297) | 0.75 ± 0.17 | 4.0 ± 0.04 (n=423) | 0.16 ± 0.05 (n=140) |
| yMTs | 0.51 ± 0.14 | 0.95 ± 0.01 (n=363) | 0.13 ± 0.03 | 1.5 ± 0.02 (n=284) | 1.08 ± 0.24 | 3.95 ± 0.04 (n=497) | 0.3 ± 0.1 | 4.6 ± 0.04 (n=200) | 0.37 ± 0.13 (n=30) |
| αΔCTT/βΔCTT | 0.22 ± 0.10 | 0.61 ± 0.01 (n=531) | 0.1 ± 0.04 | 0.56 ± 0.01 (n=375) | 1.1 ± 0.32 | 2.2 ± 0.03 (n=508) | 0.3 ± 0.1 | 4.2 ± 0.03 (n=665) | 0.3 ± 0.12 (n=30) |
| α1a/βΔCTT | 0.22 ± 0.10 | 0.98 ± 0.03 (n=388) | 0.1 ± 0.03 | 0.61 ± 0.01 (n=472) | 0.39 ± 0.18 | 0.8 ± 0.01 (n=472) | 0.27 ± 0.16 | 1.8 ± 0.04 (n=374) | 0.7 ± 0.3 (n=138) |
| α1aΔY/βΔCTT | 0.25 ± 0.12 | 0.64 ± 0.01 (n=296) | 0.1 ± 0.05 | 0.91 ± 0.01 (n=386) | 1.16 ± 0.27 | 1.91± 0.02 (n=266) | 0.29 ± 0.14 | 4.1 ± 0.02 (n=553) | 0.32 ± 0.18 (n=54) |
| αΔCTT/βII | 0.64 ± 0.15 | 1.52 ± 0.01 (n=431) | 0.09 ± 0.03 | 1.30 ± 0.02 (n=527) | 1.36 ± 0.3 | 4.8 ± 0.05 (n=318) | 0.24 ± 0.12 | 3.7 ± 0.05 (n=366) | 0.49 ± 0.2 (n=68) |
| α1a/βII | 0.52 ± 0.12 | 1.84 ± 0.01 (n=329) | 0.1 ± 0.03 | 1.22 ± 0.01 (n=424) | 0.74 ± 0.2 | 2.2 ± 0.02 (n=354) | 0.26 ± 0.13 | 2.4 ± 0.02 (n=342) | 1.52 ± 0.5 (n=130) |
| α1aΔY/βII | 0.50 ± 0.14 | 1.34 ± 0.01 (n=471) | 0.1 ± 0.03 | 1.13 ± 0.02 (n=466) | 1.32 ± 0.29 | 5.6 ± 0.08 (n=445) | 0.27 ± 0.12 | 4.4 ± 0.03 (n=465) | 0.51 ± 0.2 (n=92) |
| α1aE452C/βIIE435C | 0.58 ± 0.16 | 1.12 ± 0.01 (n=137) | 0.09 ± 0.03 | 1.28 ± 0.02 (n=343) | 0.8 ± 0.22 | 2.0 ± 0.02 (n=477) | 0.22 ± 0.1 | 2.5 ± 0.04 (n=367) | 0.9 ± 0.27 (n=60) |
| α1a-10E/βII-3E* | 0.52 ± 0.22 | 1.0 ± 0.01 (n=708) | ND | ND | 1.26 ± 0.25 | 3.7 ± 0.04 (n=298) | ND | ND | ND |
| α1a-10E/βII-10E* | 0.54 ± 0.17 | 1.61 ± 0.02 (n=199) | 0.1 ± 0.03 | 1.32 ± 0.01 (n=358) | 1.21 ± 0.27 | 3.7 ± 0.05 (n=392) | 0.4 ± 0.15 | 4.3 ± 0.05 (n=411) | 0.9 ± 0.4 (n=84) |
| αΔCTT/βIII | 0.62 + 0.18 | 0.60 ± 0.01 (n=263) | 0.1 ± 0.03 | 2.1 ± 0.03 (n=215) | 1.31 ± 0.26 | 3.2 ± 0.05 (n=190) | ND | ND | ND |
| αΔCTT/βIIIΔK | 0.64 ± 0.17 | 1.35 ± 0.02 (n=170) | ND | ND | ND | ND | ND | ND | ND |
| α1aE452C/βIII | 0.63 ± 0.18 | 0.54 ± 0.02 (n=154) | ND | ND | ND | ND | ND | ND | ND |
| α1a-3E/βIII* | 0.48 ± 0.19 | 1.04 ± 0.01 (n=199) | ND | ND | ND | ND | ND | ND | ND |
| α1a-10E/βIII* | 0.60 ± 0.20 | 1.50 ± 0.02 (n=153) | ND | ND | ND | ND | ND | ND | ND |
| αΔCTT/βIV | 0.65 ± 0.17 | 1.26 ± 0.02 (n=185) | 0.08 ± 0.02 | 1.31 ± 0.03 (n=215) | 1.33 ± 0.25 | 3.3 ± 0.041 (n=247) | 0.2 ± 0.07 | 4.7 ± 0.1 (n=162) | 0.52 ± 0.15 (n=54) |
| αΔCTT/βV | 0.58 ± 0.15 | 1.17 ± 0.02 (n=107) | 0.07 ± 0.02 | 1.53 ± 0.02 (n=228) | ND | ND | ND | ND | ND |
| αΔCTT/βVI | 0.53 ± 0.18 | 0.71 ± 0.02 (n=145) | 0.08 ± 0.02 | 1.31 ± 0.02 (n=108) | ND | ND | ND | ND | ND |
| αΔCTT/βVII | 0.25 ± 0.11 | 0.91 ± 0.01 (n=214) | 0.09 ± 0.03 | 1.02 ± 0.03 (n=141) | ND | ND | ND | ND | ND |
| αΔCTT/βVIII | 0.64 ± 0.16 | 1.01 ± 0.01 (n=353) | 0.08 ± 0.03 | 1.35 ± 0.02 (n=214) | ND | ND | ND | ND | ND |
| α1a/βIV | ND | ND | ND | ND | ND | ND | ND | ND | 1.6 ± 0.5 (n=104) |
| α1aΔY/βIV | ND | ND | ND | ND | ND | ND | ND | ND | 0.53 ± 0.18 (n=72) |
| α1aE452C/βIVE435C | ND | ND | ND | ND | ND | ND | ND | ND | 0.91 ± 0.3 (n=54) |
| α1a-10E/βIV-10E* | ND | ND | ND | ND | ND | ND | ND | ND | 0.89 ± 0.3 (n=74) |
3 or 10 glutamate peptide crosslinked to cysteine light tubulin mutants as indicated.