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. 2014 Jul 31;10(7):e1004292. doi: 10.1371/journal.ppat.1004292

Figure 3. PIA/PNAG production in the non-mucoid variant.

Figure 3

A. Real-time RT-PCR analysis of icaC mRNA transcript was performed. Bars represent the mean fold difference in transcript amount relative to strain MN8 with n = 3, and error bars indicate the standard deviations. Statistical comparison (unpaired t test) of MN8m and JB12 versus MN8 gave a P value of <0.001. B. EDTA extractions of surface-associated PIA/PNAG, and spent medium from cultures (secreted polysaccharide) were blotted and probed with PNAG antiserum (MN8m extracts and spent medium were diluted 1∶100).