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. 2014 Jul 31;10(7):e1004292. doi: 10.1371/journal.ppat.1004292

Figure 5. Evidence of icaC tandem repeat variation in clinical S. aureus isolates, and the effect on PNAG production.

Figure 5

A. Nucleotide sequence alignment of the region of repeats identified in icaC, comparing mucoid MN8m and non-mucoid isolate JB12 with two published sequences of S. aureus clinical isolates, NRS63 and NRS264. B. PNAG slot blot analysis of S. aureus strains MN8, MN8Δica, MN8m, JB12, NRS63, and NRS264 probed with PNAG-specific polyclonal antiserum. Due to the very high levels of PNAG produced by MN8m, cell surface extracts from this strain were diluted 1∶100 prior to immobilization on membrane.