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The fefe mutant of Cucumis melo does not upregulate iron (Fe) uptake genes. (a) Root ferric-chelate reductase activity (± SD) after 3 d of treatment. Figure shows a representative experiment of wild type (WT) and three separate experiments of fefe roots over a range of Fe concentrations. (b) Quantitative real-time RT-PCR for FRO1, FIT, Nramp1, and IRT1 in WT and fefe mutant roots. Relative expression as normalized to ubiquitin and WT at 25 μM Fe.
