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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: J Cell Physiol. 2014 Nov;229(11):1802–1816. doi: 10.1002/jcp.24633

Fig. 1. Lung barrier function is not disrupted in lambs with increased pulmonary blood flow.

Fig. 1

Protein extracts prepared from the peripheral lung tissue of 4-week old shunt and age matched control lambs were subjected to immunoblot analysis using a specific antiserum raised against vascular endothelial growth factor (VEGF), thrombin, and transforming growth factor (TGF)-β1. Densitometric analysis indicated that VEGF (A), thrombin (B), and TGF-β1 (C) protein levels were significantly increased in shunt compared to control lambs. The accumulation of water in lung tissue was used to determine barrier function in vivo. The wet to dry lung weight ratio of shunt lambs was not different than age matched control lambs (7.1 ± 1.4 in 12 shunts vs. 6.7 ± 0.5 in 11 control lambs; p = 0.3) (D). Total RNA was isolated from the peripheral lung tissues of 4-week old shunt or control lambs. Messenger RNA levels for Sox18 were elevated in shunt lambs compared to age matched control lambs, as determined by SYBR Green real-time RT-PCR analysis (E). The protein levels of Sox18 were significantly increased in peripheral lung tissue of shunt lambs (F). Values are mean ± SEM, n=4–12. *p<0.05 vs. sham operated control.