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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: J Cell Physiol. 2014 Nov;229(11):1802–1816. doi: 10.1002/jcp.24633

Fig. 7. Claudin-5 expression is increased in shunt lambs.

Fig. 7

Total RNA was isolated from peripheral lung tissues of 4-week old shunt or sham-operated control lambs. Messenger RNA levels for Claudin-5 were significantly increased in shunt lambs compared to age matched control lambs, as determined by SYBR Green real-time RT-PCR analysis (A). In addition, immunoblot analysis demonstrated that the protein levels of Claudin-5 were significantly elevated in the peripheral lung tissues of shunt lambs as compared to sham-operated control lambs (B). Values are mean ± SEM, n=5, *p<0.05 vs. sham-operated control.