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. 2014 Jun 26;42(13):8621–8634. doi: 10.1093/nar/gku539

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Scheme 1. — Kinetic model for the inhibition of the puromycin reaction by compound 4. Symbols: C, poly(U)-programmed ribosomes from E. coli bearing Ac[³H]Phe-tRNA^Phe at the P-site, and tRNA^Phe at the E-site; S, puromycin; P, Ac[³H]Phe-puromycin; C′, ribosomal complex not recycling; I, compound 4.