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. 2014 Jun 21;42(13):8330–8342. doi: 10.1093/nar/gku551

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Expression of CstF-64 and τCstF-64 in wild type and Cstf2 gene-trap interrupted Cstf2^E6 and Cstf2^G6 mouse embryonic stem cells. (A) Schematic representation of insertion of the gene-trap β-galactosidase-neomycin (Bgeo) fusion protein in the first (Cstf2^E6) and third (Cstf2^G6) introns of the Cstf2 gene in the two respective ESC lines. The gene-trap consists of a splice acceptor (SA) site and polyadenylation (PA) signal. The lighter shade represents the open reading frame of Cstf2 mRNA. (B) Western blot analysis of CstF-64 and τCstF-64 expression in WT (lane 1), Cstf2^E6 (lane 2) and Cstf2^G6 ESCs (lane 3). (C) Relative mRNA expression analysis of Cstf2 and Cstf2t mRNAs in the wild type, Cstf2^E6 and Cstf2^G6 ESC lines. ** denotes P < 0.01 and *** denotes P < 0.001.