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. 2014 Jun 21;42(13):8330–8342. doi: 10.1093/nar/gku551

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — CstF-64 and τCstF-64 are necessary for normal 3′ end processing of replication-dependent histone mRNAs. (A) Western blot analysis of CstF-64 and τCstF-64 expression in WT (lane 1), Cstf2^E6 ESCs transfected with scrambled siRNA (lane 2) and Cstf2^E6 ESCs transfected with siRNA specific for Cstf2t gene (lane 3). (B) Relative mRNA expression analysis of polyadenylated and total Hist1h3c histone mRNA in wild type ESCs (orange), Cstf2^E6 cells transfected with scrambled siRNA (blue) or Cstf2^E6 cells transfected with siRNA against Cstf2t gene (brown). (C) Western blot analysis of CstF-64 and τCstF-64 expression in WT ESCs either transfected with scrambled siRNA (lane 1) or Cstf2 gene specific siRNA (lane 2). (D) Relative mRNA expression analysis of polyadenylated and total Hist1h3c histone mRNA in wild type ESCs transfected with scrambled (orange) or Cstf2 gene specific siRNA (blue). (E) Western blot analysis of CstF-64 expression in WT ESCs (lane 1), Cstf2^E6 ESCs (lane 2) and Cstf2^E6 ESCs ectopically expressing CstF-64 (lane 3). (F) Corresponding relative mRNA expression analysis of polyadenylated and total Hist1h3c histone mRNA. * denotes P < 0.05 and ** P < 0.01.