Skip to main content
. 2014 Jul 2;42(13):8473–8485. doi: 10.1093/nar/gku565

Figure 1.

Figure 1.

SMARCA5 functions in transcription-coupled repair. (A) Immunoblots show reduced SMARCA5 expression levels in HeLa cells stably expressing shRNA and U2OS cells treated with siRNA against SMARCA5. Tubulin was used as loading control. (B) SMARCA5 depletion sensitizes cells to UV. Colony survival of HeLa cells stably expressing shRNA against SMARCA5 or CSB following UV irradiation. The percentage of surviving cells is plotted against the applied UV-C dose (J/m2). (C) DNA repair synthesis (UDS) after UV irradiation (16 J/m2), determined by EdU incorporation, as a measure for GG-NER, in wild-type primary fibroblasts (C5RO) (>100 cells for each sample) treated with siRNA. Plotted are, respectively, control (set at 100% UDS), XPC and SMARCA5 siRNAs. (D) Recovery of RNA synthesis (RRS), as a measure for TC-NER, determined by EU incorporation 16 h after UV irradiation (0 and 6 J/m2) in HeLa cells (>100 cells) treated with, respectively, control (set at 100% at 0 J/m2), CSB and SMARCA5 siRNAs. Error bars denote standard error of the mean. The results of all experiments were confirmed at least twice.