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. 2014 Jul 9;42(13):8379–8388. doi: 10.1093/nar/gku574

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — (A, left) Annealing between radiolabeled quadruplex hras-1 and complementary Y-strand in binding buffer (‘Materials and Methods’ section) added with 100 mM KCl. The amount of MAZ-GST was 3 μg; (A, right) Annealing between radiolabeled quadruplex hras-2 and complementary Y-strand in binding buffer added with 30 mM KCl. The amount of MAZ-GST was 3 μg. EMSA has been carried out with 12% PAGE in TB at 20°C; (B) Plots showing the fraction of duplex formed in the presence or absence of MAZ-GST, as a function of time.