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. 2014 Jul 9;42(13):8379–8388. doi: 10.1093/nar/gku574

Figure 8.

Figure 8.

(A) Structure of ATPD-1 and scheme of the transfection experiment. T24 cells were treated with ATPD-1 for 24 h, then transfected with luciferase vector. Right panel shows the dual luciferase assay in T24 cells treated with increasing amounts of ATPD-1 (2.5 and 5 μM) and transfected with wild-type pHRAS-luc or mutant Mut-1, Mut-2, Mut-A, Mut-B, Mut-C and Mut-D; (B) EMSA showing the binding of MAZ-GST (2 μg) to 20 nM quadruplex hras-1 in binding buffer containing increasing amounts of ATPD-1 (r = 0, 1, 2, 4, 6 and 8); (C) FRET-melting of 200 nM hras-1 after overnight incubation in 50 mM KCl and ATPD-1 at r = 0, 1, 2, 4, 6, 8; (D) FRET-melting curves of hras-1 (200 nM) treated with ATPD-1 (r = 0, 1, 2, 4, 6, 8) and MAZ-GST (400 nM); (E) as in D, but with 800 nM MAZ-GST. The curves in (B), (D) and (E) have been normalized to the fluorescence at 80°C (or 85°C).