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. 2014 Jul 9;42(13):8648–8662. doi: 10.1093/nar/gku579

Figure 7.

Figure 7.

Transfected PS-ASOs can seed the formation of paraspeckle-like foci in the absence of NEAT1. (A) Co-localization of PS-ASOs with P54nrb in NEAT1 RNA-absent, nuclear paraspeckle-like foci in certain cells (yellow arrows), as indicated by IF staining of P54nrb and RNA-FISH of NEAT1 RNA in HeLa cells. The ASO signal was intensified for one cell (dashed box) to indicate the co-localization of PS-ASOs with P54nrb and NEAT1 RNA in a canonical paraspeckle (white arrow). (B) PS-ASOs can induce the formation of paraspeckle-like foci in mESCs, as indicated by IF staining of PSF and mESC marker Sox2. (C) Nuclear retention of mouse CTN-RNA by paraspeckle-like foci. RNA-FISH of mouse CTN-RNA and NEAT1 RNA in MEF cells transfected with ASO ISIS454395 indicated the localization of CTN-RNA in both canonical paraspeckles containing NEAT1 RNA (white arrow, and signal intensity profile for a region marked with a dash line) as well as in paraspeckle-like foci lacking NEAT1 RNA (yellow arrows, and signal intensity profile).