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. 2014 Jul 9;42(13):8692–8704. doi: 10.1093/nar/gku597

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — She2- and Loc1-dependent co-transcriptional recruitment of Puf6 on bud-localized mRNAs. (A) Genome browser snapshots of Puf6 enrichment over the regions surrounding the MID2 and SRL1 genes. The bottom part shows zoom-in views and arrows represent amplicons used for qPCR analysis. Amplicon lengths for MID2 and SRL1 are 92 bp and 90 bp, respectively. (B) ChIP-qPCR confirmation of Puf6-TAP recruitment on MID2 and SRL1, in WT, she2 and loc1 strains. Values presented are mean ± SEM (N = 3) (*P < 0.05, **P < 0.01, unpaired t-test).