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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Gut. 2014 Jan 31;63(11):1711–1720. doi: 10.1136/gutjnl-2013-305964

Figure 1. Procedure for geographic mapping of the cell lineages within the human stomach.

Figure 1

The three entire donor stomachs were divided into regions of 0.5 cm height X 2 cm wide to embed in paraffin blocks and 1 mm cores from the paraffin blocks were excised and assembled into tissue arrays. These arrays were stained with cell lineage-specific antibodies, and then the cell numbers per core were determined using a digital quantitation system (Ariol SL-50). Finally, the distribution of cell lineages was displayed in three-dimensional projections of the stomach.