Table 2.
Sequences of polymerase chain reaction primers and the length of amplicons
| Gene | Accession number | Primers (5′–3′) | Size (bp) |
|---|---|---|---|
| COL1A1 | AF153062 | TGGTTCTCCTGGCAAAGAT ATCACCGGGTTCACCTTTA |
232 |
| COL3A1 | XM_535997 | ACAGCAGCAAGCTATTGAT GGACAGTCTAATTCTTGTTCGT |
156 |
| Fibronectin | CFU52106 | GATGACTCGTGCTTCGAC CTTCTCGCCAATCTTGTAGTT |
183 |
| Lubricin | XM_547439 | GGCCCGCTATCAATTACC ACTTCATTATGGAGGAAACCTTTA |
157 |
| TGFβ-1 | NM_001003309 | ACCATTCATGGCATGAACC CAGATCCTTGCGGAAGTC |
174 |
| GAPDH | AB038240 | TATGATTCTACCCACGGCAA CAGTGGACTCCACAACATAC |
154 |
COL1A1 = collagen type 1, alpha 1; COL3A1 = collagen type 3, alpha 1; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; TGFβ-1 = transforming growth factor-beta 1.