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. 2014 Aug 1;28(15):1641–1646. doi: 10.1101/gad.247205.114

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© 2014 Millay et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — Analysis of myomaker transcript after tamoxifen-induced satellite cell deletion. (A) Diagram showing the myomaker targeted alleles used in this study. The myomaker^loxP allele contains loxP sites flanking exon 2, and the myomaker^LacZ/+ allele has a LacZ cassette in intron 1 that inhibits exon 1 splicing with exon 2. Exon 1s refers to the first exon of a short isoform of myomaker that is annotated but not highly conserved within mammalian species. The arrows below the schematic denote primer sets used in B and C. (B) Both control (n = 3, myomaker^LacZ/+;Pax7-CreER^T2 or myomaker^loxP/+;Pax7-CreER^T2) and myomaker^scKO (n = 4, myomaker^LacZ/loxP;Pax7-CreER^T2) mice were treated with tamoxifen, injured with CTX, and analyzed by qPCR for the presence of exons 1 and 2 and exons 3 and 4 using the indicated primer sets. Data are presented as mean ± SEM. (*) P < 0.05. (C) PCR with primer set 3 shows a shorter amplicon in myomaker^scKO muscle due to the Cre-mediated excision of exon 2.