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. 2014 Aug 1;28(15):1695–1709. doi: 10.1101/gad.244434.114

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© 2014 Knight et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — Effect of Rap1 on TF recruitment and transcription noise strength. (A) Fhl1 and Hmo1 promoter occupancy (qPCR-ChIP) on the indicated RPL30 promoter alleles. Data are represented as mean ± SEM. (B) Histone H3 occupancy (qPCR-ChIP) on the indicated RPL30 promoter alleles. Data are represented as mean ± SEM. (C–F) RPG promoter occupancy of Rap1-AID (C), Ifh1-Myc (D), Fhl1-Myc (E), and Hmo1 (F) at the indicated times following auxin-induced depletion of AID-tagged Rap1. Data are plotted as auxin relative to vehicle treatment and normalized to t = 0. Data are represented as mean ± SEM. (G) The intrinsic and extrinsic noise strength of the wild-type and mutant versions of the RPL30 promoter were measured by microscopy using diploid yeast cells containing both RPL30 promoter-YFP reporter and RPL30 promoter-CFP reporter constructs.