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. 2014 Aug 1;28(15):1710–1720. doi: 10.1101/gad.246397.114

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© 2014 Elsholz et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 2. — Autophosphorylation inactivates EpsAB. (A) Immunoblot of His₆-tagged EpsB from the wild-type strain (BAE318) and strains producing EpsB^D81A/D83A (BAE451) and EpsB^Y225F/Y227F (BAE450) grown in biofilm-inducing medium. The blot was probed with anti-His₆ antibodies (top row) and anti-phosphotyrosine antibodies (bottom row). (B) Colony wrinkling on biofilm-inducing medium for strains NCBI 3610 (WT), BAE252 (∆epsAB), BAE320 (∆epsAB amyE∷epsAB^Y225F/Y227F), and BAE406 (∆epsAB amyE∷epsAB^Y225E/Y227E).