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. 2014 Jul 10;47(1):32. doi: 10.1186/0717-6287-47-32

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© Castro et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Inhibitory effect on NO• and PGE2 production by ether (A and C) and dichloromethane (B and D) fractions from Cryptostegia grandiflora on RAW 264.7 cells stimulated with LPS (10 μg/mL) for 24 h. 1400 W (10 μM) and Rofecoxib (10 μM) were employed as positive controls. Nitrite concentration in the culture medium was measured with the Griess reagent. PGE2 production was determined using a competitive ELISA kit (R&D Systems, USA). Experiments were carried out in triplicate and results show the mean ± S.E.M. (n = 12). **p < 0.01, ***p < 0.001, compared with LPS treated group.