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. 2014 Aug 1;5:395. doi: 10.3389/fmicb.2014.00395

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Copyright © 2014 Chander, Koelbl, Puckett, Moser, Klingele, Liles, Carrias, Mead and Schoenfeld.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of E. ictaluri LAMP reactions by LFD. Sensitivity was determined by testing 100-fold dilutions of E. ictaluri S97-9773 (strain 1) and E. ictaluri 219 (strain 2) in LAMP (upper panel). Dilutions over the same range of E. coli and E. tarda were tested using for the same reactions to confirm specificity (lower panel). Positive reaction is indicated by the appearance of two red lines, one at “Control” and other at “Test.” Negative reaction is indicated by appearance of only one red line at “Control.”