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Detection of DNA targets: Staphylococcus aureus (Staph. aureus), Bacillus atrophaeus (BAT), and Porcine circovirus (PCV-2) using OmniAmp LAMP assays. (A) For each pathogen, 10-fold serial dilutions of extracted DNA were prepared in water and used as template in LAMP reaction. Amplification was performed on a real time thermocycler in triplicate with average TTRs shown for each dilution. (No amplification is indicated by “**”). (B) PCV-2 LAMP products were separated on 2% agarose gel.
