Table 1.
List of targets for which LAMP assays were developed using OmniAmp polymerase.
| Pathogen | Genome | Target gene | Incubation temp. (°C) | Source |
|---|---|---|---|---|
| MS2 phage (MS-2) | RNA | Replicase protein (MS2g4) | 70 | ATCC |
| Swine influenza virus (SIV) H1N1 | RNA | Matrix (M) | 72 | University of Minnesota, St. Paul, MN |
| Porcine circovirus-2 (PCV-2) | DNA | Capsid protein (ORF 2) | 70 | |
| West Nile virus (WNV)—NY 2001-6263 | RNA | Envelope glycoprotein | 72 | ZeptoMetrix, Buffalo, NY |
| Edwardsiella ictaluri | DNA | Repetitive element | 70 | Auburn University, AL |
| Bacillus atrophaeus (BAT) | DNA | ATP synthase, β-subunit | 68 | Steris Corporation, OH |
| Staphylococcus aureus MSSA | DNA | Carbamate kinase (arcC) | 68 | ZeptoMetrix, Buffalo, NY |
| *Ebola virus (EBoV)—Zaire | RNA | Glycoprotein (GP) | 72 | Galveston National Lab, TX (RNA only) |
| *Crimean-Congo hemorrhagic fever virus (CCHFV) | RNA | Nucleoprotein (S) | 68 | |
| Bovine viral diarrhea virus (BVDV)—type I | RNA | 5′-UTR | 70 | Wisconsin Veterinary Diagnostic Laboratory, Madison, WI |
*
RNA extracts were provided by Galveston National Laboratory, TX and were certified for use in BSL II facility.