Table 4.
Agonist effects of N-acyl amide mixtures on calcium mobilization in BV-2 microglia.
| N-acly amide mixtures 10 μ M | BV-2 Microglia |
|---|---|
| N-acyl alanine | 69.48 ± 3.18 |
| N-acyl aspartic acid | 12.73 ± 0.82 |
| N- acyl beta-alanine | 15.33 ± 1.16 |
| N- acyl GABA | 119.56 ± 2.72 |
| N- acyl glycine | 22.91 ± 1.32 |
| N-acyl isoleucine | 8.31 ± 1.17 |
| N-acyl leucine | 56.47 ± 1.62 |
| N-acyl methionine | 33.00 ± 1.84 |
| N-acyl phenylalanine |  |
| N-acyl proline | 102.66 ± 2.04 |
| N-acyl serine | 12.41 ± 1.35 |
| N-acyl threonine | 12.27 ± 1.00 |
| N-acyl tryptophan | 4.83 ± 1.04 |
| N-acyl tyrosine | 19.74 ± 1.24 |
| N-acyl valine | 61.33 ± 2.17 |
| DMSO | 9.09 ± 0.84 |
| Capsaicin (500 nM) | 9.60 ± 2.54 |
| PAF | 49.60 ± 2.71 |
| Inonomycin | 223.92 ± 5.81 |
Far left column lists the N-acyl amide family or control compound that was used as the challenge compounds for each screen. Values shown are the change in relative fluorescence units of Fura2AM from the 30 s baseline and represent the area of the curve of analysis of 190 s post injection (see example on Figure 2. Those values in bold black are significantly higher from those of the vehicle injection, whereas those in bold gray are significantly lower p ≤ 0.01.