Fig. 4.

Bone marrow-derived macrophages and dendritic cells infected with L. monocytogenes: cellular responses. a Intracellular viable counts were determined 24 h p.i., MOI 0.2, after treatment of cells with 10 µg/ml gentamicin (dendritic cell population from wildtype compared to properdin-deficient mice: p < 0.005). b IFN-γ released into the supernatant of infected cells (24 h) was measured by ELISA (dendritic cell population from wildtype compared to properdin-deficient mice: p < 0.01). c Nitric oxide release of infected cells (24 h) and controls, determined by the Griess reaction (dendritic cell population from wildtype compared to properdin-deficient mice: p < 0.01). Representative of two independent experiments set-up in triplicate