Fig. 7.

Properdin-deficient and wildtype mice (n = 9 each genotype) were injected with passaged L. monocytogenes (1 × 10e⁶) and culled at humane endpoint. Survival was plotted against 100 % and was higher in wildtype compared to properdin-deficient mice (p < 0.05, a viable counts were determined by serial dilution from livers homogenised in PBS; homogenisation in dH₂O gave the same distribution (b). IFNγ was determined by ELISA using sera pooled from mice with identical score of disease severity (lethargic, n = 2 each genotype (p < 0.0005). c IL-17A mRNA expression was quantified from spleens of uninfected mice (n = 2, p < 0.005) and of mice of equal severity of listeriosis (hunched, piloerect), culled 29 h p.i. (n = 1 WT, n = 2 KO, p < 0.005). Note the increased overall abundance of expression in infected mice (d)