Fig. 1.

Construction and genotyping of FtMt^−/− mice. a Schematic of the FtMt locus and targeting vector, the arrows indicate the PCR primers for genotyping. b Genotyping gel of mice wild type (+/+), heterozygous (+/−), and homozygous (−/−) for the inactivated FtMt allele. In the M lane, a DNA molecular weight ladder was loaded. The size of the expected PCR amplicons is indicated. c RT-PCR analysis of testis RNA extracted from wild type (+/+), heterozygous (+/−), and homozygous (−/−) mice and amplified with primers for FtMt, beta-galactosidase (β-gal, encoded by the Lac-Z gene), and the housekeeping transcript HPRT1. d Immunoblotting of protein extracts from the testis of wild type (+/+), heterozygous (+/−), and homozygous (−/−) mice overlaid with antibodies for mouse FtMt, β-gal, and GAPDH as loading calibrator. e Immunoblotting of protein extracts from various tissues of wild type (+/+) and homozygous (−/−) mice, overlaid with antibodies for mouse FtMt. (f,g) Immunohistochemical detection of FtMt in the testis of wild type (f) and FtMt(−/−) (g) mice. Bar 50 μm