Fig. 4.

Indices of oxidative stress and iron status in the heart of Dox-treated mice. Heart extracts from 8-week-old wild-type (+/+) and FtMt-deficient mice (−/−) treated with Dox 15 mg/kg or saline and sacrificed after 4 days were analyzed. Oxidative stress was evaluated by measuring cardiac lipid peroxidation with the TBARS assay (a), HO-1 mRNA expression by qRT-PCR (b) and oxidized proteins by oxyblot (c). Ferritin was detected by Western blotting of non-denaturing PAGE using specific antibodies for H (FTH) and L ferritin (FTL); for evaluation of ferritin iron, the gels were stained with Prussian blue and then the color enhanced using DAB and H₂O₂ (Ft-iron) (d). Coomassie blue stain of the gels is shown for load calibration. At least five animals per group were analyzed, two representative samples are shown