Table 1.

Primers and Taqman assays used

Genotyping primers
B5-Fwd	5′-ACGCGTCGACCTTGTGTTAGTAATTCAGCC-3′
mT4-Rev	5′-CAGAGTATGTAAGTCCAGCAGC-3′
LacZ-Rev	5′-GGGACGACGACAGTATCGGCCT-3′
RT-PCR primers
BGAL-FwdNew	5′-GCACGGTTACGATGCGCCCA-3′
BGAL-RevnNew	5′-GCGCTGGAGTGACGGCAGTT -3′
mT1-For	5′-TATTTCCTTCGCCAGTCCCTG-3′
mT4R-Rev	5′-CAGAGTATGTAAGTCCAGCAGC-3′
mHPRT1-For	5′-GCTTGCTGGTGAAAAGGACCTCTCGAAG-3′
mHPRT1-Rev	5′-CCCTGAAGTACTCATTATAGTCAAGGGCAT-3′
TaqMan gene	Assay ID
HO-1	Mm00516005_m1
BNP	Mm01255770_g1
p53	Mm00519571_m1
Rn18s	Mm03928990_g1

The genotyping primers were used to verify the presence of FtMt gene or of LacZ gene in the FtMt locus. RT-PCR primers were used for identification of the transcripts of FtMt, β-Gal, and HPRT1 in the testis of the mice. The TaqMan gene expression assays were used to quantify HO-1, BNP, p53, and Rn18s transcripts in the heart of the treated mice