Figure 6.

Gene expression of CAMs and its impact on aggregate size. (A) The gene expression patterns of cell adhesion molecules (CAMs) in chimera vs. in clone. Each row represents a gene and each column represents an experiment set. The x-axis represents time, and y-axis represents M, where M = log₂(Cy3/Cy5 signal intensity). Red and blue lines represent gene expression patterns in chimera and clone, respectively. Asterisks represent significant differences identified by ANOVA. (B) Gene network that coordinately regulates aggregate size in D. discoideum development. Positive regulators of aggregate size are listed in the left panel, and negative regulators are on the right. Genes in orange showed up-regulation in expression level in chimera vs. in clone at the aggregate stage, while genes in green were down-regulated in expression. The expression of genes in white was not significantly different in chimera vs. clone, and the expression of genes in grey was not detected on the D. discoideum microarray.