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. 2014 Jun 24;289(31):21413–21422. doi: 10.1074/jbc.M114.581124

FIGURE 1.

FIGURE 1.

FGFR1 inhibits PDP1 via phosphorylation at Tyr-94. A, schematic representation of PDP1. Five identified FGFR1-direct tyrosine phosphorylation sites are shown. B, Western blot (WB) detecting tyrosine phosphorylation (p) of purified GST-FLAG-PDP1 from cells co-expressing FGFR1 wild type (WT) or an FGFR1 kinase dead form (KD) using a pan phospho-Tyr (pTyr) antibody. C, active rFGFR1 (p-FGRF1) directly phosphorylates purified GST-FLAG-PDP1 at tyrosine residues in an in vitro kinase assay. D, purified FLAG-tagged PDP1 variants were incubated with rFGFR1 followed by PDP1 phosphatase assay in the presence of purified PDC. PDP1 phosphatase activity was assessed by the altered phospho-Ser-293 levels of PDHA1 (p-PDHA1(S293)). ns, not significant. Error bars indicate mean ± S.D. *, p < 0.05.