FIGURE 9.

Time courses for Rubisco activation measured from purified recombinant wild-type and mutant I165A and A47V/M331A enzymes. Percentages of maximal specific activity (measured with respective enzymes that had been fully activated) were plotted against the time allowed for activation after being inactivated by the formation of the enzyme-inhibitor complex. The maximal specific activity values measured were 2.2 μmol/min/mg for the wild type, 0.24 μmol/min/mg for mutant I165A, and 0.05 μmol/min/mg for mutant A47V/M331A. Data are representative of two identical experiments carried out with two separate enzyme preparations, which gave similar results.