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. 2014 Jun 18;289(31):21433–21450. doi: 10.1074/jbc.M114.578625

TABLE 2.

Kinetic properties of recombinant R. palustris Rubisco enzymes purified from either autotrophically grown R. capsulatus or E. coli

RrcbbM WT refers to R. rubrum form II wild-type enzyme.

Enzyme Ωa (VcKo/VoKc) kcata Kca Koa Ko/Kcb KRuBPa
s1 μm μm μm
Wild type 12 ± 1 4.9 ± 0.2 104 ± 22 277 ± 15 2.7 10 ± 2
A47V 11 ± 1 0.9 ± 0.2 99 ± 34 82 ± 31 0.8 6 ± 2
A47V/M331A 6 ± 1 0.2 ± 0.1 433 ± 61 697 ± 77 1.6 20 ± 6
I165V 4 ± 1 1.3 ± 0.2 178 ± 47 391 ± 45 2.2 35 ± 9
RrCt 13 ± 1 6.7 ± 0.7 111 ± 28 270 ± 71 2.4 8 ± 2
RrcbbM WT 13 ± 1 4.3 ± 0.5 125 ± 12 143 ± 3 1.1 5 ± 2

a Values are the means ± S.D. (n − 1) of at least three separate enzyme preparations.

b Calculated values.