TABLE 2.
Kinetic properties of recombinant R. palustris Rubisco enzymes purified from either autotrophically grown R. capsulatus or E. coli
RrcbbM WT refers to R. rubrum form II wild-type enzyme.
| Enzyme | Ωa (VcKo/VoKc) | kcata | Kca | Koa | Ko/Kcb | KRuBPa |
|---|---|---|---|---|---|---|
| s−1 | μm | μm | μm | |||
| Wild type | 12 ± 1 | 4.9 ± 0.2 | 104 ± 22 | 277 ± 15 | 2.7 | 10 ± 2 |
| A47V | 11 ± 1 | 0.9 ± 0.2 | 99 ± 34 | 82 ± 31 | 0.8 | 6 ± 2 |
| A47V/M331A | 6 ± 1 | 0.2 ± 0.1 | 433 ± 61 | 697 ± 77 | 1.6 | 20 ± 6 |
| I165V | 4 ± 1 | 1.3 ± 0.2 | 178 ± 47 | 391 ± 45 | 2.2 | 35 ± 9 |
| RrCt | 13 ± 1 | 6.7 ± 0.7 | 111 ± 28 | 270 ± 71 | 2.4 | 8 ± 2 |
| RrcbbM WT | 13 ± 1 | 4.3 ± 0.5 | 125 ± 12 | 143 ± 3 | 1.1 | 5 ± 2 |
a Values are the means ± S.D. (n − 1) of at least three separate enzyme preparations.
b Calculated values.