Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jun 16;289(31):21451–21462. doi: 10.1074/jbc.M114.560821

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 10. — Effects of ZIP2 knockdown on differentiation of HDK1 in a three-dimensional cultured epidermis. a, immunostaining images of the three-dimensional cultured epidermis constructed using HDK1 (negative control group). The expression of KRT14 was confirmed in the basal layer of the three-dimensional cultured epidermis (top panels). The expression of IVL was confirmed in the upper layer of the three-dimensional cultured epidermis (bottom panels). b, immunostaining images of the three-dimensional cultured epidermis constructed using HDK1 (ZIP2 knockdown group). The expression area of KRT14 was expanded in the ZIP2 knockdown group compared with the negative control group (top panels). The expression area of IVL was smaller in the ZIP2 knockdown group than in the negative control group (bottom panels). The presence of cell nuclei was confirmed around the cornified layer (top and bottom panels). The arrowheads indicate nuclei that are retained in cells in the cornified layers. White dotted lines mark the three-dimensional cultured epidermis constructed using HDK1. Scale bars = 50 μm.