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. 2014 Jun 16;289(31):21451–21462. doi: 10.1074/jbc.M114.560821

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Differentiation induction of HDK1. a, differentiation process of HDK1. The differentiation of HDK1 was induced by elevating the extracellular calcium levels of KSFM to 3.0 mm. b, expression analysis of IVL by Western blotting. The differentiation of HDK1 was induced by elevating the calcium levels of KSFM to 1.5 and 3.0 mm. Cells were collected on day 4 of the culture and analyzed for the expression of IVL. c, immunostaining images of HDK1 stained for the IVL antibody after differentiation induction. The expression of IVL was confirmed after a 4-day induction. Scale bars: 200 μm (a) and 200 μm (c).