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. 2014 Jun 16;289(31):21451–21462. doi: 10.1074/jbc.M114.560821

FIGURE 6.

FIGURE 6.

Effects of ZIP2 knockdown on the differentiation of HDK1. a, gene expression analysis of ZIP2 and IVL on day 4 of differentiation induction. The expression of IVL was down-regulated significantly by the knockdown of ZIP2. b, images of HDK1 in which ZIP2 was knocked down by siRNA. On day 4 of differentiation, HDK1 did not exhibit a cobblestone morphology by the knockdown of ZIP2. c, immunostaining images of ZIP2 knockdown HDK1 stained with the IVL antibody on day 4 of differentiation. The expression of IVL was down-regulated significantly by the knockdown of ZIP2. d and e, intracellular zinc levels in HDK1 (NT and si-pool) on day 4 of differentiation. The ratio of FluoZin-3 high cells was decreased by ZIP2 knockdown. Scale bars = 200 μm (b) and 200 μm (c). Data are mean ± S.D. of three experiments. **, p < 0.01; significantly different from the control.