FIGURE 11.

Sonicated αSyn reduces the Ca²⁺ retention of brain mitochondria without measureable enhancement of ROS production. A, representative traces of the Ca-Green 5N fluorescence of vehicle-treated (black) and 2 μm sonicated αSyn-treated brain mitochondria (red) under complex I conditions. αSyn-treated mitochondria were able to buffer fewer Ca²⁺ additions to the solution. B, quantification of the relative CRC of vehicle- and sonicated αSyn-treated mitochondria. Error bars, S.D. from eight independent experiments. *, p < 0.05 using Student's t test. C, ROS production from vehicle-treated (black) and sonicated αSyn-treated (red) mitochondria (left) was measured using the dye Amplex Red concurrently with CRC (right). D, the mean slopes of the increase in Amplex Red fluorescence during Ca²⁺ accumulation were quantified. Despite its ability to reduce CRC, sonicated αSyn did not lead to a significant increase in ROS production in this system. Error bars, S.D. of five independent experiments.