FIGURE 8.
αSyn does not affect mitochondrial parameters in the absence of exogenous Ca2+. A, comparison of mitochondrial suspensions incubated with vehicle control (black) or 1 μm sonicated αSyn fibrils (red) under complex I conditions in the absence of exogenous Ca2+. The addition of sonicated αSyn resulted in no alteration of steady state ΔΨm, Ca2+ flux, oxidation state of pyridine nucleotides, or membrane swelling. Alamethicin (Ala) was added to induce complete swelling after 1 h. Arrows, the addition of mitochondria. Traces are representative of four independent experiments. B and C, representative swelling traces of mitochondria incubated with either vehicle (black) or 2 μm sonicated fibrils (red) under complex I conditions and treated with 1 μm (B) or 10 μm (C) phenylarsine oxide (PhAsO). αSyn incubation did not sensitize mitochondria to undergo mPTP-related swelling in the presence of phenylarsine oxide.