FIGURE 5.

The ^NLSDMP1 transgene has no rescue effects on Dmp1-null mice but the ^SPDMP1 transgene fully rescues the Dmp1-null bone phenotype. a–c, compared with ^SPDMP1, ^NLSDMP1 has no effect on bone shape and secondary ossification (a, Alizarin red/Alcian blue staining, arrows), bone porosity and mineralization reflected by micro-CT images (b, arrowheads), and Goldner staining (c, red indicating a lack of mineral). d–h, the ^NLSDMP1 had no apparent effect on the osteocyte (Ocy)-lacunocanalicular system (d, S.E.), and expression of decorin (e, an inhibitory factor in mineralization), OSX (f, a marker for Ob), E11 (g, an early marker for osteocytes), and SOST (h, a marker for mature osteocytes).