FIGURE 2.

Peptidase and collagenase activity of autoactivated cathepsin K. A, time-dependent autoprocessing of pro-hrCatK (4 μm) to mature hrCatK in the presence (+) or absence (−) of 18 μm C4-S at pH 5.0. The volumes from reactions in A were transferred to the subsequent assays. B and C, peptidase activity (B) and collagenase activity (C) of the activated hrCatK enzyme produced in A in a time-dependent manner were demonstrated as described under “Experimental Procedures.” D, sequence mapping to identify N termini of products from autoprocessing of pro-hrCatK (4 μm) after 1 and 2 h of incubation at 37 °C in the presence of C4-S at pH 5.0.