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Autoprocessing of the pro-domains from wild type and mutant cathepsin K isoforms demonstrates trans-mechanism of cleavage. Processing of catalytically inactive mutant pro-hrCatK(C139G) (A) or pro-hrCatK(WT) (B) at 4 μm was demonstrated by incubating with increasing concentrations (0–200 nm) of the preactivated enzyme (hrCatK-Act) without (−) and with (+) 18 μm C4-S. The reaction mixture was incubated at 37 °C for 6 h.
