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Effect of CD-loop specific peptide and CD-loop mutation on reduction of MtbHbN by FdR. A, reduction of MtbHbN (8 μm) in the presence of CD loop-specific and nonspecific peptide (40 μm) by FdR (1 μm). A, control reaction with HbN-FdR; B, HbN-FdR with CD loop peptide; C, HbN-FdR with nonspecific peptide. B, reduction profile of HbN CD loop mutants by FdR recorded as absorbance change at 420 nm due to conversion of ferric HbN into ferrous state and binding of CO.
