TABLE 1.
Kinetic parameters for in vitro heme reduction of MtbHbN and its mutants
| Protein complex | Km | kcat | Heme iron reduction | Ref. |
|---|---|---|---|---|
| μm | min−1 | μm/min | ||
| HbN-FdR | 19.9 | 13 | 6.2 | This study |
| HbN-KshBa | This study | |||
| HbNS47W-FdR | 20.9 | 9.9 | 5.1 | This study |
| HbNG48I-FdR | 19.4 | 8 | 4.2 | This study |
| HbNT49W-FdR | 20.0 | 13.2 | 6.2 | This study |
| HbNΔPre-A-FdR | 16.3 | 1.5 | 0.9 | This study |
| VHbb-FdR | 12.4 | 7 | 2.2 | This study |
| Mb-FdR | 15 | 3.5 | 1.3 | Our estimation |
| Mb-FdR | 17 | 5 | 2 | 13 |
a Not determined due to extremely slow reaction.
b VHb (Vitreoscilla hemoglobin) as reported elsewhere (19).