Specific interaction of glucose and inhibitors at 37 °C. Data of one typical experiment are presented for each cantilever, probed in different conditions: initial condition (first column), block condition (second column), and washout condition (third column). Values are the mean ± S.E. for n = 2000–3000, where 2–3 different cells were probed at each condition. The specificity of the recognition events could be demonstrated by a significantly reduced binding probability in the presence of phlorizin when compared with control levels (initial condition) with p < 0.005. After a careful washout procedure the interaction increased again. amino-phl, 3-aminophlorizin.