TABLE 1.

Kinetic property of glucose, hexyl-glucose, and aminophlorizin binding to rbSGLT1

The values were obtained from the experiments depicted in Fig. 6. k_off of thioglucose on AA-PEG₅₀₀₀ at 37 °C compared with k_off of thioglucose on AA-PEG₅₀₀₀ at 10 °C and 25 °C and k_off of aminophlorizin on mal-PEG₁₃₀₀ at 37 °C when compared with k_off of aminophlorizin on mal-PEG₁₃₀₀ at 10 °C and 25 °C were significantly different. x_ß of thioglucose on AA-PEG₅₀₀₀ at 37 °C when compared with x_ß of the other ligands was significantly different.

SGLT1 sensors	Width of the energy barrier, xß			Dissociation rate constant, koff
	10 °C	25 °C	37 °C	10 °C	25 °C	37 °C
	Å			s−1
Thio-glc on AA-PEG5000	3.96 ± 0.08	4.99 ± 0.1	6.24 ± 0.08a	0.60 ± 0.05	0.83 ± 0.07	0.26 ± 0.02b
hex-glc on mal-PEG1300	3.48 ± 0.05	3.18 ± 0.1	2.75 ± 0.1	0.41 ± 0.03	0.50 ± 0.07	0.40 ± 0.07
Amino-phl on mal-PEG1300	3.23 ± 0.06	4.28 ± 0.1	2.93 ± 0.04	0.34 ± 0.03	0.54 ± 0.07	1.00 ± 0.06b

^a p < 0.005.

^b p < 0.001.